Meth detection from sperm-

This is how drugs affect your swimmers. Men produce literally millions of sperm a day, but it is still common for a healthy man with a fertile partner to take a year to conceive. But there is solid evidence that drug use can seriously disadvantage your little swimmers — and damage your chances of starting a family. Some studies are more conclusive than others and, often they only examine heavy drug users, but it still makes for some sobering reading. Some argue that marijuana should be legal, but what does the drug do to your sperm count?

Meth detection from sperm

The results showed that MA decreases sperm concentration, progressive motility and increases abnormal sperm morphology in a dose-dependent manner. Altered protamine expression and diminished spermatogenesis: what is the link? Fertil Steril It is shown that there is a positive relationship between sperm chromatin defects and cell death or apoptosis The Meth detection from sperm of the fetus in this fluid may prolong exposure to harmful drugs or metabolites. Meth detection from sperm Understanding the effect of male age on fertility has also become important in public Dumbledor gay because of a great number of men are choosing to father a child at older age. Drugs, the brain, and behavior: The pharmacology of drug use disorders. The range of saliva pH is typically much narrower 6. Schmidt L, Minister K, Helm P Infertility and the seeking of infertility treatment in a representative population. However, the harmful effects can be reversed if you cut back on the beers.

Rectal incontenance. Introduction

Wperm employers may elect a ten-panel drug test that also includes any of the above plus:. It is important to notify the laboratory or MRO of any medications currently in use, including prescriptionover-the-counter or herbal medications. Here are 11 surprising ways to live longer. He has been writing since Pre-employment workplace drug testing usually requires the applicant to give a urine sample, but may also infrequently require blood, saliva, sweat, or hair. The Content on this Site is presented in a summary fashion, and is intended to be used for educational and entertainment purposes only. If the sample is non-negative, an additional Meth detection from sperm GC-MS analysis is performed eMth a separate portion the biological sample. Diabetes Type 1 Type 2 Prevention. All times are GMT Guest Statistics on mn teenage drivers a year ago from meth Meeth cocks gotten really hard as hard as ive seen it maybe harder and ive cummed higher than my head and alot like 5 times a night.

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Methamphetamine MA was shown to have harmful effects on male reproductive system. Normal saline was injected in sham group and no medications were used in control group. To evaluate sperm chromatin abnormalities, the aniline blue, toluidine blue and chromomycine A3 were used.

For sperm morphology, Papanicolaou staining was done. There was a significant increase in rate of aniline blue, toluidine blue, and chromomycine A3 positive spermatozoa in high dosage group. In a similar manner, there was an increase in rates of acridine orange, TUNEL and sperm chromatin dispersion positive sperm cells in high dosage group with respect to others. In recent years, methamphetamine MA has been attractive and road drug in several countries, because of its quite easy manufacture and low price versus to another drugs 1.

MA is an illegal psycho stimulant drug profitable to amphetamine type 2. MA is a strongly addictive drug with a high probability of addition which is absorbed slowly for a long period of time, consequently for hr 3.

It is usually used by young and teenagers who are in the age of reproduction 4. In fact, young population experience MA for fun and improvement of sexual function at first times 5. Earlier, many studies proved negative effects of morphine and cocaine, but today consumption of synthetic drugs including amphetamines or MA is increased in developed and developing countries 6 - 8.

The exact mechanism by which MA leads to male reproductive system dysfunctions is not completely understood. There are many studies indicating the deleterious effects of MA on reproductive organs 9. Experimental studies on rodents suggest some mechanisms of MA action on male fertility potential including altered hormonal profiles, oxidative stress, DNA damage of spermatozoa, and abnormal spermatogenesis 10 , It is also demonstrated that MA adversely affects on seminiferous epithelium including degeneration and apoptosis of germ cells It is also suggested that MA affects male reproductive function at multiple levels due to its effects on the endocrine control of spermatogenesis 13 , Recently, it has been reported that MA decreases normal sperm morphology and count, as well as increase apoptotic cells in seminiferous tubules 15 , In a study conducted by Zuloaga et al , histopathological and histomorphometric alterations in seminiferous tubules have been reported in MA-treated animals Evaluation of sperm nuclear chromatin is a noticeable approach for male fertility investigations.

During spermatogenesis, sperm chromatin is compacted more and more due to histones replacement at first by testis-specific nuclear proteins, then by transitional proteins and finally by protamines Disulphide bonds of protamine molecules are crucial for sperm nuclear compaction and stabilization. It is believed that this kind of nuclear compaction protects sperm genome from damages including oxidative stress, elevated temperature and acid-induced DNA denaturation Oxidative stress OS is considered as an important cause of male infertility leading to an increase in sperm DNA fragmentation.

Imbalance between reactive oxygen species ROS production and semen antioxidant ability results OS It is generally accepted that ROS affects sperm chromatin condensation and also may have harmful effects on sperm motility, morphology and fertilization ability To the best of our knowledge, there are no study that investigated the effects of MA on sperm chromatin condensation and DNA integrity.

Therefore, we designed the present study to investigate the effects of different doses of MA on sperm count, motility, and morphology and sperm chromatin integrity in male mouse as an experimental model.

They were fed with normal pellet diet and water adlibitum. Normal saline was injected daily in sham group, but, the control mice did not receive any medication. The percentages of progressive fast and slow movements , non-progressive and immotile spermatozoa were calculated For assessment of sperm morphology, the Papanicolaou staining was done. Briefly, the smears were fixed by ethanol-ether for 5 min and then they were stained with PAP staining solutions according to WHO guidelines Different forms of sperm morphology were determined including normal, double head, pin head, amorphous head, coiled mid piece, coiled tail, bent tail, and cytoplasmic droplet Figure 1-A.

The AR was assessed by double staining method. The smears were prepared after two times washing rpm, 2 min. The slides were stained with Bismarck brown 0.

After washing, smears were dehydrated in ethanol series and rinsed in xylene Red or pink staining of the acrosomal region determined as acrosome-intact spermatozoa. AB staining is a cytochemical assay for detection of remained histones in the process of sperm chromatin remodeling In this staining, the spermatozoa with unstained nucleus are considered as normal and spermatozoa with dark blue nuclei are counted as abnormal ones Figure 1-B.

The air-dried smears were fixed in a solution of ethanol-acetone at 4 o C for 30 min. Hydrolysis of smears was performed by HCl 0. Then, TB dye solution 0. Finally, the slides were rinsed in distilled water and dehydrated with ethanol and xylene at room temperature for 3 min Spermatozoa with normal chromatin are seen colorless but sperm cells with mild, medium and sever chromatin abnormality were seen in dark blue, violet and purple respectively Figure 1-C.

CMA3 staining was used for indirect assessment of protamine deficiency. The prepared slides were evaluated under fluorescent microscope with nm filter. The bright yellowish spermatozoa were considered as positive CMA3, while others without brightness were considered as negative CMA3 with normal protamine.

In the next step, they were immersed in 0. The apoptotic sperm cells were presented as percentage in each sample. This assay is used for detection of sperm DNA damage. Then, coverslip was removed and slide was embedded in 0.

Each slide was immersed in lysis solutions 1 and 2 sequentially. The time of lysis solution 1 0. Finally, each slide was rinsed in wright stain for 10 min. The small, medium and large halos around sperm heads were determined in comparison with core width of spermatozoa.

The small halo showed high DNA fragmentation and the medium and large ones showed moderate and without DNA fragmentation in spermatozoa respectively 33 Figure 2-C. All protocols were performed according to the national institute of health guide for the care and use of laboratory animals NIH Publications No. For statistical analysis, we used Statistical Package for the Social Sciences, version According to table I , although, the sperm count showed a decreasing trend in medium and high dosage groups when compared to low dosage and control one, but it was not statistically significant.

Normal morphology was significantly decreased in medium and high dosage groups compared to other groups. Due to the high prevalence of MA abuse in young populations especially in men who are in reproductive period, the present study was planned to estimate the specific deleterious effects of this central nervous system stimulant as an addictive drug on sperm parameters and chromatin integrity in male mice.

The results showed that MA decreases sperm concentration, progressive motility and increases abnormal sperm morphology in a dose-dependent manner. Moreover, sperm chromatin packaging and apoptosis could be influenced by different dose of MA.

The effects of MA on sperm chromatin also appear to be dose-depended as similar drugs which had more negative effects in high doses. Our findings were similar to the study by Alavi and colleagues which have demonstrated that frequent consumption of high dosage of MA may reduce the number of sperm in the epididymis tail and has negative effects on reproductive system in male rats Additionally, several experimental studies showed that administration of different dose of MA caused decreasing in the sperm count and normal morphology 35 , On the other hand, Lin and colleagues reported many defects in testicular tissue and spermatogenesis cell lines including the elevation of apoptotic cells and destructive changes in seminiferous tubules in male rats which were treated by MA.

In addition, they presented that sperm count and motility were decreased in MA administration animals It is generally accepted that in male fertility evaluations, the standard routine sperm analysis is not enough, because it does not show different forms of sperm chromatin and DNA damages.

So, in the present study, we focused on molecular and cytochemically-based assays to determine the effects of MA on sperm chromatin condensation and apoptosis in mouse as a good experimental model. It is shown that there is a positive relationship between sperm chromatin defects and cell death or apoptosis Similar to our study, Yamamoto and colleagues reported that administration of MA reduces the spermatogenesis and increases apoptosis in spermatogonial cell line in rat Additionally, Alavi and his colleagues demonstrated that repeated administration of MA in male rats enhances apoptosis of spermatogonia and primary spermatocytes in seminiferous tubules.

In fact, they discussed that decreasing in sperm count and normal morphology and apoptotic cell increasing in seminiferous tubules may be due to the MA toxicity in sperm germ line and testicular tissue Another recent study has reported a reduction of reproductive ability in animals that were administered by MA Also, in a sex hormones assessment, it has been suggested that any alternation in serum testosterone during the MA treatment can affect the spermatogenesis and it has a main role in activation of apoptosis in spermatogonial cells Although in present study we can not report any evidence about the mechanism of harmful characteristics of MA on gonadal hormones and risky effect on spermatogenesis, but it could be mentioned that reduction in sperm chromatin quality was dependent on toxic effects of MA and hormonal profile defects.

Recently, it was shown that MA can induce apoptosis in testicular germ cells and epididymal spermatozoa in laboratory animals AB and CMA3 are two main tests for identifying sperm chromatin packaging and protamine deficiency of spermatozoa It is confirmed that the lower amounts of nuclear protamines affect the sperm chromatin packaging and as a result the sperm quality The present study was also the first study to the evaluation of the sperm acrosome in MA administration animals.

There was a significant difference between the percentage of spermatozoa with intact acrosomes in experimental and control groups. In conclusion, MA can increase abnormal spermatozoa with inadequate chromatin and DNA integrity in a dose-dependent manner and it is important to aware the young male people from deleterious effects of this drug on reproductive indices.

This article extracted from M. Mojdeh Sabour. The authors have no financial or nonfinancial conflicts of interest. National Center for Biotechnology Information , U. Int J Reprod Biomed Yazd. Mojdeh Sabour , M. Find articles by Mojdeh Sabour. Find articles by Arezoo Khoradmehr. Find articles by Seyyed Mehdi Kalantar.

Find articles by Amir Hossein Danafar. Find articles by Marjan Omidi. Find articles by Iman Halvaei. Find articles by Ali Nabi. Find articles by Saeed Ghasemi-Esmailabad.

Your not as interested in sex as when you are on it. However, workplace drug testing is now common in general for many U. Step 1 Buy a black light. Consumer News. Observe the clothing.

Meth detection from sperm

Meth detection from sperm

Meth detection from sperm

Meth detection from sperm

Meth detection from sperm

Meth detection from sperm. Related Topics

You said he Can't ejaculate? Is that always the case or is it off and on? Alot of us men don't like to go to the doctors and get our junk checked because if a doctor says it dosn't work then some of us feel like we hae lost our manhood. Be concerned and show it but don't make it a BIG deal we have ego's to protect. There is also other things like stress from work or home which can effect it.

He probably feels some stress because of it also. I know first hand about how he feels thats why me and my ex broke up because right before we got together i was using meth and quit for a few months for her but still had the lingering effects meth takes 1 year minnimum to be completely out of your system even though i told her she still didn't understand why I couldn't get erect and accused me of still using meth.

So please don't listen to the first reply u get here just TALK to him and try not to pressure him it puts alot of stress on a relationship. Guest over a year ago from meth my cocks gotten really hard as hard as ive seen it maybe harder and ive cummed higher than my head and alot like 5 times a night. Maybe it's bcause we were shooting it? And it was many yrs ago when quality was better. All it takes me and my tongue ring is about 4 minutes and u will melt all over the place!! Still to this day, i have the same issue he is having.

As for ejaculation He can ejaculate. Your going to have to have sex for maybe hours in order for it to happen. If you suck him. He will get hard. So when you change into a sex positiion does he get limp as you change into another position? When you quit meth. Your not as interested in sex as when you are on it. All fellow tweekers honestly should know what im talking about. Check it out..

HE is a tweeker. Here is another way. Listen to him go pee. For real though. Look at his pee colore. Is it dark and yellow? The heck with it. Look for stains on them that you can see with your naked eye first to determine which spots you should take a closer look at. Scan closely the clothes you have gathered. Hold the light about six inches from the clothing and move it slowly across the piece entirely, focusing especially on the spots that seemed to be present before to your naked eye.

Observe the clothing. Semen will show up as a light yellow color when scanned with a black light. This should give you an answer as to whether semen is on clothing or not. Josh Repka has had honors bestowed on him throughout his academic experience, and has received recognition for his works in the field of English, Writing and Literacy.

He has been writing since He has been published on eHow. By: Joshua R. Step 1 Buy a black light.

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Log out of Readcube. Click on an option below to access. Log out of ReadCube. Methamphetamine has been reported to be an important drug in the field of reproductive toxicology. The aim of this study was to investigate the effects of methamphetamine administrations on sperm morphology, sperm concentration and apoptotic activity inside seminiferous tubule in male rats. Percentage of normal sperm morphology was decreased in acute group when compared with control. Apoptotic activities were most abundant in the seminiferous tubules of acute treated animals with a highly significant increase in the number of apoptotic cells per tubule.

The results suggest that methamphetamine not only works as drug of abuse in central nervous system, but also in gametogenesis of males. Volume 43 , Issue 4. The full text of this article hosted at iucr. If you do not receive an email within 10 minutes, your email address may not be registered, and you may need to create a new Wiley Online Library account.

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Get access to the full version of this article. View access options below. You previously purchased this article through ReadCube. Institutional Login. Log in to Wiley Online Library. Purchase Instant Access. View Preview. Learn more Check out. Summary Methamphetamine has been reported to be an important drug in the field of reproductive toxicology. Citing Literature. Volume 43 , Issue 4 August Pages Related Information. Close Figure Viewer. Browse All Figures Return to Figure.

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Meth detection from sperm

Meth detection from sperm